ISO-23418 › Microbiology of the food chain - Whole genome sequencing for typing and genomic characterization of bacteria - General requirements and guidance
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This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
. products intended for human consumption;
. products intended for animal feed;
. environmental samples from food and feed handling and production areas;
. samples from the primary production stage.
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Document Number
ISO 23418:2022
Revision Level
1ST EDITION
Status
Current
Publication Date
June 1, 2022
Committee Number
ISO/TC 34/SC 9