ISO-23418 Microbiology of the food chain - Whole genome sequencing for typing and genomic characterization of bacteria - General requirements and guidance

ISO-23418 - 1ST EDITION - CURRENT


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This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:

a) handling of bacterial cultures;

b) axenic genomic DNA isolation;

c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;

d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;

e) metadata capture and sequence repository deposition;

f) validation of the end-to-end WGS workflow (fit for purpose for intended application).

This document is applicable to bacteria isolated from:

.    products intended for human consumption;

.    products intended for animal feed;

.    environmental samples from food and feed handling and production areas;

.    samples from the primary production stage.

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07.100.30 (Food microbiology Including microbiology of animal feeding stuffs Animal feeding stuffs, see 65.120 General methods of tests and analysis for food products, see 67.050)

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Document Number

ISO 23418:2022

Revision Level

1ST EDITION

Status

Current

Publication Date

June 1, 2022

Committee Number

ISO/TC 34/SC 9