ASTM-E1536 › Standard Practice for Detection of Mycoplasma Contamination of Bovine Serum by Large Volume Method (Withdrawn 2014)
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Scope
1.1 This practice covers the procedures used for detection of mycoplasma contamination in serum by direct microbiological culture.
1.2 This practice does not cover procedures used for detection of mycoplasma in cell cultures.
1.3 This practice does not cover indirect methods for detection of mycoplasma contamination.
1.4 This practice does not cover methods for identification of mycoplasma cultures.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Significance and Use
Mycoplasmas of bovine origin are prevalent contaminants of cell cultures. Contamination can be detected by the large volume method.3 ,4
Heat inactivated serum need not be tested for mycoplasmas. Heating serum to 56°C for 30 min will kill mycoplasmas.
Mycoplasmas may be present in any particular lot of serum but may not be detected because of inadequate sample size; thus, negative test results do not provide absolute assurance that the test serum is free of mycoplasmas.
Keywords
mycoplasma; serum; ICS Number Code 07.100.10 (Medical microbiology)
To find similar documents by ASTM Volume:
11.06 (Biological Effects and Environmental Fate; Biotechnology)
To find similar documents by classification:
07.100.10 (Medical microbiology Laboratory medicine, see 11.100)
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Document Number
ASTM-E1536-00(2006)
Revision Level
2000 R06 EDITION
Status
Cancelled
Modification Type
Withdrawn
Publication Date
Jan. 1, 2006
Document Type
Practice
Page Count
2 pages
Committee Number
E55.04