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This document specifies a method for the determination of the substances in leather which are soluble in dichloromethane. This method is applicable to all types of leather. Not all fatty and similar substances can be extracted from leather with organic solvents; they may be in part soluble and partly bound to the leather. On the other hand, the solvent can dissolve non-fatty substances, for example sulfur and impregnants, both of which cause difficulty in the determination of the acid value and saponification value of the fat. This document includes two techniques for extraction of the fatty substances: 1) extraction using the Soxhlet apparatus; and 2) extraction using a pressurized extraction system. As the extraction is frequently done in conjunction with determination of the free fatty acid content of the leather, a suitable procedure for determination of the free fatty acids extracted by this method is included. The apparatus and technique described in this method are also suitable for the extraction by solvents other than dichloromethane (although the temperature conditions may need to be varied for high pressure extraction).