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This International Standard specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 µg/kg. The method has been validated for maize containing 24,5 µg/kg, for peanut butter containing 8,4 µg/kg, and for raw peanuts containing 16 µg/kg of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.