SS-EN-17477 › Algae and algae products - Identification of the biomass of microalgae, macroalgae, cyanobacteria and Labyrinthulomycetes - Detection and identification with morphological and/or molecular methods
SS-EN-17477
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2021 EDITION
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CURRENT
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This document specifies a method for the detection and identification of microalgae, macroalgae, cyanobacteria and Labyrinthulomycetes by using morphological methods and/or molecular methods. The morphological methods in this document are applicable to harvested wet biomass and to harvested dried unground biomass from microalgae, macroalgae, cyanobacteria and Labyrinthulomycetes that have been grown and/or harvested for further processing and/or use. The molecular methods in this document are applicable to harvested wet biomass and to harvested dried and/or ground biomass from microalgae, macroalgae, cyanobacteria and Labyrinthulomycetes that have been grown and/or harvested for further processing and/or use. This document describes a toolbox, consisting of several identification methods that can be chosen according to the applicability and purpose of the identification: - morphological methods based on observation and referring to scientific literature on taxonomy: - macroscopic identification; - light microscopic identification. - molecular methods for sequencing and blasting of sequences: - 16S rDNA sequencing; - 18S rDNA sequencing; - rbcL DNA sequencing; - ITS sequencing; - COX1 gene sequencing; - tufA gene sequencing. This document does not deal with genetic purity of the biomass or quantification of the identified taxa. This document is not suitable for the analysis of highly processed biomass with highly degraded DNA where the fragmentsÂ’ length are not sufficient for amplification of the targets and the morphological characteristics cannot be assessed.
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Designation Name
SS-EN 17477:2021
Revision Level
2021 EDITION
Status
Current
Publication Date
Aug. 17, 2021
Language(s)
English
Page Count
36
International Equivalent
EN 17477:2021(IDT)