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This International Standard specifies two methods for the determination of aflatoxin B₁ in animal feeding stuffs. These methods can only be used for semi-quantitative determinations.

Method A is applicable to the following simple feeding stuffs:

• oilseeds and oilseed residues, and in particular groundnut, copra, linseed, soya, babassu palm;
• manioc meal;
• maize germ expeller;
• cereals and cereal products;
• pea meal;
• potato pulp and flour.

In the presence of substances interfering with the determination by method A, it is recommended that the determination be carried out in accordance with method B.

Method B is applicable to mixed feeding stuffs and to simple feeding stuffs not mentioned in 1.2.

This method is not applicable to feeding stuffs containing citrus pulp.