ISO-21446 › Infant formula and adult nutritionals - Determination of trans and total (cis + trans) vitamin K1 content - Normal phase HPLC
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This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.
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Document Number
ISO 21446:2019
Revision Level
1ST EDITION
Status
Current
Publication Date
Jan. 1, 2019
Committee Number
ISO/TC 34